DIRECTIONS FOR COLLECTION OF SEMINAL SPECIMEN VIA THE HYGENE™ COLLECTION
Although there are instructions for use of the Hygene™
Kit on its package, there are a few other instructions that you need
to be aware of before you begin to produce the specimen. Intercourse
is the preferred method of obtaining your semen sample, however, if
needed, the specimen may be collected via manual stimulation (masturbation)
or coitus interruptus (See Step 6 below).
You should remove the TYB
(yellow colored media) that you placed in the freezer before and rewarm
to body temperature by placing it in a glass of warm water for 20-30
minutes before you begin to produce the specimen. To produce the seminal
specimen you may follow the directions given by the manufacturer of
the Hygene™ Kit with some modifications as described below. Preferably,
there should be a 2-4 day period of sexual abstinence prior to the
collection to maximize the recovery of the number and quality of collected
sperm (See References 1 and 2 below).
|The Hy-Gene Kit contains a fertility condom,
funnel, transort vial and label.
- Have small, clean scissors
available prior to collection via the Hygene™ Kit method.
and dry hands and penis.
- Remove plastic wrap from carton. Holding
carton upright, use your thumb to break perforation around carton
and then remove top portion.
- Remove fertility condom from funnel
and place against top of erect penis. To prevent trapping air, hold
reservoir tip while unrolling condom onto penis.
- Proceed naturally
and refrain from penetration and ejaculation for as long as 5 minutes
in order to elicit maximum sexual stimulation (Reference 1 below).
After ejaculation, hold open end of condom to prevent slippage.
- Once erection has subsided, remove condom
being careful not to spill any semen. (If semen is to be collected
via masturbation proceed with step 7, placing semen in funnel and
into the vial, then following step 9 through step 15).
|The specimen is collected then extracted
from the condom through the funnel and into the transport vial.
- Place funnel in vial (blue top test-tube).
- Holding reservoir end of condom over funnel,
use scissors to cut a small slice in tip. Carefully squeeze all possible
semen from condom (see diagram on Hygene™ box).
- After all semen has collected
in vial, (test-tube) remove funnel and discard funnel and condom.
- Remove and dry the TYB bottle from glass
of warm water. Open the TYB bottle carefully and pore all contents
in the vial with semen and seal vial with cap. Shake gently 3-4 times
by inverting the vial.
- Complete identification label and apply
it to unprinted portion of vial (test-tube).
- Place the vial in the middle slot on the
top of the styrofoam core (where TYB was). Place the separator cover
(thin piece of styrofoam with two holes) over the test-tubes.
|The funnel is discarded and the transport
vial is sealed and labeled
Place the frozen blue cold pak on top of the separator cover.
Place the lid on the styrofoam container and seal it with a strong
shipping tape to secure the container lid to the box. Circle the
container with your shipping tape in the slots on the outside of
the box. Also, tape the circumference where the lid meets the box
to prevent cool air from escaping.
- Place the styrofoam container
in the corrugated shipping box provided and seal it securely with
your shipping tape. Make sure the furnished pre-addressed shipping
label (Federal Express USA Airbill) is properly attached to the shipping
box. For specific instructions for shipping, please refer to Shipping
Information. Hygene Kit contents Semen Specimen Collection Discard
- Zavos, PM, Characteristics of human ejaculates
collected via masturbation and a new Silastic seminal fluid collection
device. Fertil. Steril. 43:491, 1985.
- Zavos, PM, Seminal parameters
of ejaculates collected from oligospermic and normospermic patients
via masturbation and at intercourse with the use of a Silastic seminal
fluid collection device. Fertil. Steril. 44:517, 1985.
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